FB2024_01 , released February 22, 2024
Assay: MassSpec_Casas-Vila_life_cycle_Am
Open Close
General Information
Name
MassSpec_Casas-Vila_life_cycle_Am
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0003432
Project
Data Provider
Title
Mass spectrometry of proteins from D. melanogaster, Oregon-R strain, adult male (1 week post-eclosion).
Accessions
    Biosample Source
    Overview
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation

    Population cages of wild-type, fertilized females were maintained at 25oC. Embryos were collected on apple juice agar plates in 2 hr windows, aged at 25oC for the required time, then dechorionated. Early larval collections were obtained from 2 hr embryo collections that were appropriately aged. Later larval and pupal stages were collected directly from flasks at the indicated time points. Adult flies were collected either within 4 hr of eclosion, or as one week old flies. Samples were snap-frozen. For each time point, samples were collected in quadruplicate.

    Biosamples used (1)
    Biosample
    Type
    Title
    D. melanogaster, Oregon-R strain, adult male (1 week post-eclosion), source for protein.
    Assay Details
    Key genes
    Protocol

    Snap-frozen samples were mechanically lysed by bead milling in lysis buffer with protease inhibitor. Homogenates were collected by centrifugation and proteins were acetone precipitated. Protein pellets were resuspended in sample loading buffer containing DTT, boiled 10 min, sonicated 10 min, then separated on 4-12% Bis/Tris gel. Proteins were digested in-gel and mass spec performed as described in Kappei et al., 2013 (PMID:23685356).

    Mode of Assay

    Peptides were separated by nanoflow liquid chromatography coupled to a Q Exactive Plus mass spectrometer (Thermo). The instrument was operated in the data-dependent mode.

    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    MassSpec_Casas-Vila_life_cycle_Am
    Name Synonyms
    Mass spectrometry of proteins from D. melanogaster, Oregon-R strain, adult male (1 week post-eclosion).
    Secondary FlyBase IDs
      References (1)