FB2024_01 , released February 22, 2024
Recombinant Construct: PBac{PB}
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General Information
Symbol
PBac{PB}
FlyBase ID
FBtp0016553
Feature type
transgenic_transposable_element
Component Allele(s)
Size
7.257
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

The 48bp FRT sites that flank the w+mPB.FRT marker in the PBac{PB} element can efficiently recombine in the presence of FLP recombinase, removing the marker and regenerating a single 48bp FRT site. However, the 48bp site is significantly less efficient for longer distance recombination reactions than the longer FRT sites commonly used in constructs designed for generation of chromosomal deficiencies.

PBac{PB} comprises a complete piggyBac element with the open reading frame interrupted by a w+mPB.FRT marker flanked by short (48bp) FRT sites.

A diagram of the PBac{PB} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

Construct components
Component
Use(s)
Marker(s)
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
(bases )
Segments and Size
Total Size

7.257

Left end
Right end
 
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (600)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Secondary FlyBase IDs
    References (21)