FB2024_01 , released February 22, 2024
Recombinant Construct: M{vas-int.Dm}
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General Information
Symbol
M{vas-int.Dm}
FlyBase ID
FBtp0036669
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component
Use(s)
Component allele phiC31\intDm.vas
Transgenic product class
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Description

A vas promoter drives expression of phiC31:int. The phiC31:int sequence has been engineered to have the codon bias of Drosophila melanogaster. The construct also has vas 3'UTR sequences.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

Generated by phiC31\int-mediated integration of p3xP3-EGFP.vas-int.Dm.attB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct.

The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP3xP3.PB marker, followed by another P1\loxP site, followed by a Avic\GFPE.3xP3 marker, followed by another P1\loxP site, followed by phiC31\intDm.vas, which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP3xP3.PB and Avic\GFPE.3xP3 markers by cre-mediated recombination.

Other than the altered integrase coding region, M{vas-int.Dm} is identical to M{vas-int.B}.

Stocks (80)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Secondary FlyBase IDs
    References (5)