A vas promoter drives expression of phiC31:int. The phiC31:int sequence has been engineered to have the codon bias of Drosophila melanogaster. The construct also has vas 3'UTR sequences.
The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP3xP3.PB marker, followed by another P1\loxP site, followed by a Avic\GFPE.3xP3 marker, followed by another P1\loxP site, followed by phiC31\intDm.vas, which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP3xP3.PB and Avic\GFPE.3xP3 markers by cre-mediated recombination.
Generated by phiC31\int-mediated integration of p3xP3-EGFP.vas-int.Dm.attB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct.
The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP3xP3.PB marker, followed by another P1\loxP site, followed by a Avic\GFPE.3xP3 marker, followed by another P1\loxP site, followed by phiC31\intDm.vas, which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP3xP3.PB and Avic\GFPE.3xP3 markers by cre-mediated recombination.
Other than the altered integrase coding region, M{vas-int.Dm} is identical to M{vas-int.B}.