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General Information
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| Term |
two hybrid |
ID (Ontology) |
MI:0018 (Molecular Interactions) |
| Definition |
The classical two-hybrid system is a method that uses transcriptional activity as a measure of protein-protein interaction. It relies on the modular nature of many site-specific transcriptional activators (GAL 4) , which consist of a DNA-binding domain and a transcriptional activation domain. The DNA-binding domain serves to target the activator to the specific genes that will be expressed, and the activation domain contacts other proteins of the transcriptional machinery to enable transcription to occur. The two-hybrid system is based on the observation that the two domains of the activator need to be non-covalently brought together by the interaction of any two proteins. The application of this system requires the expression of two hybrid. Generally this assay is performed in yeast cell, but it can also be carried out in other organism. The bait protein is fused to the DNA binding molecule, the prey to the transcriptional activator.[ PubMed:10967325 PubMed:12634794 PubMed:1946372 ] |
| Also Known As |
"2 hybrid" ; "2-hybrid" ; "2H" (for all, see Synonyms field below) |
| Comment |
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Links to Extrernal Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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two hybrid (all annotations which use CV term, excluding "NOT" statements) | 3290 |
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Relationships