FlyBase:Vectors and Constructs

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Genomic Insertion Collections

Gene Disruption Project Constructs

P{PZ} lacZ enhancer trap
P{lacW} lacZ enhancer trap
P{GT1} gene trap
P{SUPor-P}
P{EP} mobile activating element
P{EPg} mobile activating element
P{EPgy2} mobile activating element
P{Mae-UAS.6.11} mobile activating element
PBac{PB} (Exelixis)
PBac{GAL4D,EYFP} GAL4 binary enhancer trap
Mi{ET1} GAL4 binary enhancer trap
Mi{MIC} GFP gene trap, φC31 target

Drosophila Gene Search Project Constructs

P{GSV1} mobile activating element
P{GSV2} mobile activating element
P{GSV3} mobile activating element
P{GSV6} mobile activating element
P{GSV7} mobile activating element

Deletion Generators

P{XP} recombination
PBac{RB} recombination
PBac{WH} recombination
P{RS5} recombination
P{RS3} recombination
PBac{5HPw[+]} recombination
PBac{3HPy[+]} recombination
P{wHy} hobo mobilization

Protein Trap Constructs

P{PTT-GA} GFP gene trap
P{PTT-GB} GFP gene trap
P{PTT-GC} GFP gene trap

Constructs Used for Other Collections

P{A92} lacZ enhancer trap
P{lArB} lacZ enhancer trap
P{lwB} lacZ enhancer trap
P{hsneo}
P{ry11}
P{GawB} GAL4 binary enhancer trap
PBac{SAstopDsRed} Tni\piggyBac mutator
pP{MF3} VDRC-1 dsRNA construct collection vector
pVALIUM10 TRiP-1 dsRNA construct collection vector

Genome Project Cloning Vectors

cDNA Cloning Vectors

pOT2
pOTB7
pCDNA-SK[+]
pCR2.1
pFLC-I
pSport1-Tag21

BAC Vectors for Large Genomic Clones

pBACe3.6

Basic Plasmid Cloning Vectors for P Transformation

Carnegie 20 Derivatives

pP{Car20} ry+t7.2
pP{Car20.1} ry+t7.2
pP{DM30} ry+t7.2

CaSpeR Derivatives

pP{CaSpeR} w+mC
pP{CaSpeR-2} w+mC
pP{CaSpeR-3} w+mC
pP{CaSpeR-4} w+mC
pP{CaSpeR-5} w+mC

Others

pP{hsneo} Tn\neoRHsp70Bb.PS
pP{YES} y+mDint2
pP{W8} w+mW.hs
pP{YC1.8} v+t1.8
pP{Car4} -none-

Plasmid Cloning Vectors with a Promoter

pP{CaSpeR-hs/act} Hsp70
pP{CaSpeR-hs} Hsp70
pP{CaSpeR-Hsp70/SV40} Hsp70
pP{Hsp70-CaSpeR} Hsp70
pP{W8H} Hsp70
pP{CaSpeR-act(B)} Act5C
pP{CaSpeR-act(R)} Act5C
pP{hsneo-act(B)} Act5C
pP{hsneo-act(R)} Act5C
pP{GMR} GMR (eye-specific)
pP{COG} otu (oogenesis)

Plasmid Cloning Vectors with a Reporter Gene

pP{Pelican} LacZ
pP{H-Pelican} LacZ
pP{GreenPelican} GFP (vital reporter)
pP{GreenH-Pelican} GFP (vital reporter)
pP{Stinger} GFP (vital reporter)
pP{H-Stinger} GFP (vital reporter)
pP{RedH-Stinger} RFP (vital reporter)
pP{RedH-Pelican} RFP (vital reporter)
pP{YellowH-Pelican} YFP (vital reporter)
pP{CaSpeR-lacZ} LacZ
pP{CaSpeR-betagal} LacZ
pP{CaSpeR-AUG-betagal} LacZ
pP{DM79} LacZ
pP{DM26} CAT
pP{HZ50} LacZ
pP{HZ50PL} LacZ
pP{HZR} LacZ

Transposase Sources

pπ25.7wc P transposase
pP{Δ2-3} P transposase
pUChsΔ2-3 P transposase
H{PΔ2-3} P transposase
PBac{Δ2-3.Exel} P transposase
pP{HBL1} hobo transposase
pP{hsH\T-2} hobo transposase
P{hsILMiT} Dhyd\Minos transposase
P{Hsp70-PBac\T} Tni\piggyBac transposase
P{Tub-PBac\T} Tni\piggyBac transposase

Cell Ablation Vectors and Constructs

pP{RMC4}
pP{UAS-RAcs2}
P{UAS-hid}
P{UAS-hid.C}
P{hs-hid}
P{UAS-Hsap\KCNJ2.EGFP}
P{UAS-NaChBac}
P{UAS-Cbβ\DT-A.I}
P{GMR-rpr.H}
P{UAS-rpr.C}
P{GMR-grim}

FLP/FRT Constructs

pP{hsFLP}
P{70FLP}
P{hsp70-flp}
pP{neoFRT}
P{FRT(w+t1.8)}
pP{lacZ.FRT.B}
P{lacZ.Act5C.B}
pP{wg.FRT.S}
pJ35
P{ey-FLP.N}
P{ey3.5-FLP.Exel}
P{ovo-FLP.R}

GAL4/UAS Cloning Vectors

pP{UAST}
pP{UASp}
pP{UAS-C5}
pP{GawB}
pP{WIZ} RNAi, UAS
pP{PTGAL}
P{GD264}
pGaTN
pGaTB

GAL4/UAS Target Constructs

P{UAS-lacZ.B}
P{UAS-lacZ.Hsp70}
P{UAS-lacZ.N}
P{UAS-tau-lacZ.B}
P{UAS-lacZ.NUC}
P{UAS-GFP.S65T}
P{UAS-RAcs2} Cell ablation
P{AyGAL4} Mosaic analyses


Cell Culture Vectors

pMT-GAL4
pRmHa-1
pRmHa-3
pAct5CSRS
pP-Ac
pPacPL
pBLCAT3
pP{C4cat}
pSCAT0
pHGCO
p0-CAT
pcopneo

Targeted Replacement Vectors and Constructs

pP{W25} ends-out replacement
pP{W30} ends-out replacement
pP{W35} ends-out replacement
P{70I-SceI} source of Scer\SCEI endonuclease
P{hsFLP} source of Scer\FLP1 recombinase

φC31 Recombination-mediated genomic insertion

Cloning vectors for donor element

Pacman1-ApR BAC cloning vector
Pacman1-CmR BAC cloning vector
Pacman2-ApR BAC cloning vector
Pacman2-CmR BAC cloning vector
Pacman2-CmR-BW BAC cloning vector
pUASTattB plasmid cloning vector
pwP-Ex2UASTattB plasmid cloning vector; split-white system

Target elements

PBac{y+-attP-3B} target element
PBac{y+-attP-9A} target element
M{wEx3-6.attP} target element, split-white system
M{loxP.attP} target element
M{3xP3-RFP.attP} target element
M{3xP3-RFP.attP'} target element
P{CaryP} target element
P{UASTP2} target element

Integrase sources

M{vas-int.B} integrase source (source within genome)
M{vas-int.Dm} integrase source (source within genome)
M{vas-int.NLS} integrase source (source within genome)
M{nos-int.NLS} integrase source (source within genome)
M{nos-int.B} integrase source (source within genome)
pET11φC31poly(A) template for integrase mRNA production

Gateway recombinational cloning vectors (promoter; tag)

For a complete list of Gateway vectors please see the "Data from Reference" section of this page

Vector Promoter; Tag
pP{TWG} UAS; EGFP
pP{TGW} UAS; EGFP
pAGW Act5C (cell culture); EGFP
pAWG Act5C (cell culture); EGFP
pAHW Act5C (cell culture); HA1
pP{TMW} UAS; Myc
pP{TWM} UAS; Myc
pP{TWV} UAS; YFP
pP{TVW} UAS; YFP
pP{THW} UAS; HA1
pP{TWH} UAS; HA1
pP{TW} UAS; [none]
pP{PWG} UASp (female germline); EGFP
pP{PGW} UASp (female germline); EGFP