FB2024_02 , released April 23, 2024
FB2024_02 , released April 23, 2024
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Term two hybrid array ID (Ontology) MI:0397 (Molecular Interactions)
Definition Two-hybrid screening can be done in a colony array format, in which each colony expresses a defined pair of proteins. Because the particular protein pair expressed in each colony is defined by its position in the array, positive signals identify interacting proteins without further characterization, thus obviating the need for DNA purification and sequencing. The interrogation of a two-hybrid colony array usually involves a mating strategy in which every DNA binding domain hybrid (the bait) is tested against all activation domain hybrids (the preys) in a grid pattern. Arrays usually use full-length open reading frames.[ PubMed:11827624 ]
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 two hybrid array (all annotations which use CV term, excluding "NOT" statements)    1984
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  transcriptional complementation assay
   |__two hybrid
       |__two hybrid array  1984 rec.
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