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General Information
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| Term |
bimolecular fluorescence complementation |
ID (Ontology) |
MI:0809 (Molecular Interactions) |
| Definition |
The bimolecular fluorescence complementation (BiFC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two non- fluorescent fragments of a protein fluorophore such as green fluorescent protein (GFP) or its derivatives. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional flourophore that can be identified through fluorescence spectroscopy or microscopy.[ PubMed:11983170 ] |
| Also Known As |
"bifc" ; "gfp complementation" ; "green fluorescence protein complementation assay" |
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Links to Extrernal Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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bimolecular fluorescence complementation (all annotations which use CV term, excluding "NOT" statements) | 581 |
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Relationships